Precise RNA targeting with CRISPR–Cas13d – Nature Biotechnology

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  • Konermann, S. et al. Transcriptome engineering with RNA-targeting type VI-D CRISPR effectors. Cell 173, 665–676(2018).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Zhang, F. et al. Multiplexed inhibition of immunosuppressive genes with Cas13d for combinatorial cancer immunotherapy. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02535-2 (2025).

  • Tieu, V. et al. A versatile CRISPR–Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells. Cell 187, 1278–1295(2024).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Wessels, H.-H. et al. Massively parallel Cas13 screens reveal principles for guide RNA design. Nat. Biotechnol. 38, 722–727 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Morelli, K. H. et al. An RNA-targeting CRISPR–Cas13d system alleviates disease-related phenotypes in Huntington’s disease models. Nat. Neurosci. 26, 27–38 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Kelley, C. P., Haerle, M. C. & Wang, E. T. Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR–Cas13d in mammalian cells. Cell Rep. 40, 111226 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Shi, P. et al. Collateral activity of the CRISPR/RfxCas13d system in human cells. Commun. Biol. 6, 1–8 (2023).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Tong, H. et al. High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects. Nat. Biotechnol. 41, 108–119 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Li, Y. et al. The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model. Genome Biol. 24, 20 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Wei, J. et al. Deep learning and CRISPR–Cas13d ortholog discovery for optimized RNA targeting. Cell Syst. 14, 1087–1102(2023).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Guo, X. et al. Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens. Cell Genom. 1, 100001 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Wessels, H.-H. et al. Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq. Nat. Methods 20, 86–94 (2023).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Wessels, H.-H. et al. Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning. Nat. Biotechnol. 42, 628–637 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Hart, T. et al. High-resolution CRISPR screens reveal fitness genes and genotype-specific cancer liabilities. Cell 163, 1515–1526 (2015).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • DeWeirdt, P. C. et al. Optimization of AsCas12a for combinatorial genetic screens in human cells. Nat. Biotechnol. 39, 94–104 (2021).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Zhao, F. et al. A strategy for Cas13 miniaturization based on the structure and AlphaFold. Nat. Commun. 14, 5545 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • McCallister, T. X. et al. A high-fidelity CRISPR–Cas13 system improves abnormalities associated with C9ORF72-linked ALS/FTD. Nat. Commun. 16, 460 (2025).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Chen, S. et al. Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell 160, 1246–1260 (2015).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 17, 10–12 (2011).

    Article 

    Google Scholar 

  • Langmead, B., Trapnell, C., Pop, M. & Salzberg, S. L. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 10, R25 (2009).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Leek, J. T., Johnson, W. E., Parker, H. S., Jaffe, A. E. & Storey, J. D. The sva package for removing batch effects and other unwanted variation in high-throughput experiments. Bioinformatics 28, 882–883 (2012).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Kolde, R., Laur, S., Adler, P. & Vilo, J. Robust rank aggregation for gene list integration and meta-analysis. Bioinformatics 28, 573–580 (2012).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

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